Coding

Part:BBa_K1351023:Design

Designed by: Patrick Reith   Group: iGEM14_LMU-Munich   (2014-10-04)


upp cassette as counter-selection marker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 688
    Illegal SapI.rc site found at 466


Design Notes

Mutation of Freiburg Standard Restriction Sites within the genomic uppcas was needed Point Mutation of AgeI restriction site by change of G -> C at position 174 Point Mutation of EcoRI restriction site by change of C -> G at position 477



Source

Derived from pUC19_upp, AG Mascher Originally from Bacillus subtilis 168 genomic DNA


References